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Shimadzu Releases New Gradient System Protein Sequencers

Designed to provide higher sensitivity and more reliable analysis of N-terminal amino acid sequences through automated Edman degradation

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By: Kristin Brooks

Managing Editor, Contract Pharma

Shimadzu Scientific Instruments (SSI) has released the new PPSQ-51A single reactor and PPSQ-53A triple reactor gradient system protein sequencers. These instruments are designed to provide higher sensitivity and more reliable analysis of N-terminal amino acid sequences through automated Edman degradation. They’re also designed to provide an extra level of flexibility because they can detect and identify additional rare and modified amino acids. They are FDA 21 CFR Part 11 compliant when used with SSI’s LabSolutions analysis data system. 

According to the company, the PPSQ-51A/53A gradient system protein sequencers perform sequence analysis at substantially lower levels using gradient separation and a flow cell that enables high-sensitivity detection of PTH-amino acids. These systems are equipped with a high-performance detector and a solvent delivery pump that provides enhanced pumping performance. Also, high retention time stability, allows operators to more confidently identify the correct amino acid. 

PPSQ software enables simple operation, post-run processing, and reporting, according to the company. Various functions allow the reprocessing of chromatograms, the overlaying of multiple chromatograms, chromatogram subtraction and automatic estimation of amino acid sequences. In addition, the PPSQ-51A/53A sequencers provide customized reports and yield graphs for a comprehensive view of data. For regulated environments, using the PPSQ-51A/53A gradient systems with LabSolutions software enables compliance with FDA 21 CFR Part 11 guidelines for security, system policy, user management and audit trail requirements.

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